KeyTec® TR-FRET BTK WT / CRBN PROTAC Binding Assay kit
Size:
Price: $ Inquire
CAT.: A1090010D
KeyTec® TR-FRET BTK WT / CRBN PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between wild-type BTK protein and the CRBN/DDB1 complex. This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is as shown in the figure below. The KeyTec® TR-FRET Solar Eu1‑conjugated anti‑Tag1 antibody specifically recognizes the Tag1‑BTK WT protein, while the KeyTec® TR‑FRET LA2‑conjugated anti‑Tag2 antibody specifically recognizes the Tag2‑CRBN/DDB1 protein complex. The Tag1‑BTK WT protein and the Tag2‑CRBN/DDB1 protein complex can form a ternary complex mediated by the positive reference PROTAC molecule MT‑802, bringing Solar Eu and LA into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor. Detection of signal intensity at a specific wavelength (665 nm) allows determination of the PROTAC molecule‑mediated binding between BTK WT and CRBN/DDB1.
| CAT. | Description | Size |
| A1090010D | KeyTec® TR-FRET BTK WT / CRBN PROTAC Binding Assay kit | 100 tests |
|
• 1 vial of 5 µL PROTAC standard 1 MT-802 (5 mM in DMSO) • 1 vial （10 μL/vial） Tag1-BTK WT • 1 vial （20 μL/vial） Tag2-CRBN/DDB1 • 1 vial （5 μL/vial） mAb anti-Tag1 - Solar Eu • 1 vial （5 μL/vial） mAb anti-Tag2 - LA Lypholized • 1 vial （5 μL/vial） TCEP (1 M) • 2 vials （2 mL/vial） PROTAC Diluent Buffer #2 • 1 vial （2 mL/vial） PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® TR-FRET BTK WT / CRBN PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between wild-type BTK protein and the CRBN/DDB1 complex. This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is as shown in the figure below. The KeyTec® TR-FRET Solar Eu1‑conjugated anti‑Tag1 antibody specifically recognizes the Tag1‑BTK WT protein, while the KeyTec® TR‑FRET LA2‑conjugated anti‑Tag2 antibody specifically recognizes the Tag2‑CRBN/DDB1 protein complex. The Tag1‑BTK WT protein and the Tag2‑CRBN/DDB1 protein complex can form a ternary complex mediated by the positive reference PROTAC molecule MT‑802, bringing Solar Eu and LA into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor. Detection of signal intensity at a specific wavelength (665 nm) allows determination of the PROTAC molecule‑mediated binding between BTK WT and CRBN/DDB1.
| CAT. | Description | Size |
| A1090010S | KeyTec® TR-FRET BTK WT / CRBN PROTAC Binding Assay kit | 500 tests |
|
• 1 vial of 10 µL PROTAC standard 1 MT-802 (5 mM in DMSO) • 1 vial （50 μL/vial） Tag1-BTK WT • 1 vial （100 μL/vial） Tag2-CRBN/DDB1 • 1 vial （25 μL/vial） mAb anti-Tag1 - Solar Eu • 1 vial （500 tests/vial） mAb anti-Tag2 - LA Lypholized • 1 vial （120 μL/vial） TCEP (1 M) • 1 bottle （50 mL/bottle） PROTAC Diluent Buffer #2 • 1 bottle （30 mL/bottle） PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® TR-FRET BTK WT / CRBN PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between wild-type BTK protein and the CRBN/DDB1 complex. This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is as shown in the figure below. The KeyTec® TR-FRET Solar Eu1‑conjugated anti‑Tag1 antibody specifically recognizes the Tag1‑BTK WT protein, while the KeyTec® TR‑FRET LA2‑conjugated anti‑Tag2 antibody specifically recognizes the Tag2‑CRBN/DDB1 protein complex. The Tag1‑BTK WT protein and the Tag2‑CRBN/DDB1 protein complex can form a ternary complex mediated by the positive reference PROTAC molecule MT‑802, bringing Solar Eu and LA into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor. Detection of signal intensity at a specific wavelength (665 nm) allows determination of the PROTAC molecule‑mediated binding between BTK WT and CRBN/DDB1.
| CAT. | Description | Size |
| A1090010L | KeyTec® TR-FRET BTK WT / CRBN PROTAC Binding Assay kit | 5,000 tests |
|
• 1 vial of 50 µL PROTAC standard 1 MT-802 (5 mM in DMSO) • 1 vial （500 μL/vial） Tag1-BTK WT • 1 vial （1 mL/vial） Tag2-CRBN/DDB1 • 1 vial （250 μL/vial） mAb anti-Tag1 - Solar Eu • 1 vial （5000 tests/vial） mAb anti-Tag2 - LA Lypholized • 2 vials （120 μL/vial） TCEP (1 M) • 1 bottle （200 mL/bottle） PROTAC Diluent Buffer #2 • 1 bottle （120 mL/bottle） PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
English
![KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit](/uploads/file/product/A1090002S_1.jpg "KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit")
![KeyTec® TR-FRET GDP loaded KRAS[G12D]/SOS1 Binding Assay kit](/uploads/image/20260713/A1090001S.webp "KeyTec® TR-FRET GDP loaded KRAS[G12D]/SOS1 Binding Assay kit")